Monthly Archives: December 2012

12.21.12 Hydroponics Project Write Up Day 3

 Happy winter solstice! Today is the first day of winter (it sure feels like it outside too!) and it is also the day with shortest amount of daylight hours, it will only get longer from here… until June 21st that is!

Today all 4 classes worked in the computer lab to finish up on the hydroponics project paragraphs and graph. It was due today and I appreciate your dedication and hard work in completing this assignment. Remember you are now able to take your plants home so stop by after school to pick them up:

photo 1

Also, if anyone would like to take the fish home please have your parents email me their permission and the fish are free to a good home! photo 2

I wish you and your family very happy holidays and will see you next year!

 

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Hydroponics Project Write Up Day 2

For all 4 classes:

Your objective a to collect and analyze the results of your hydroponics project in order to write up your final draft due Friday.

We worked in the computer lab to complete your paragraphs and line graph using the resources found on Hydroponics Links from the page above. You will spend the rest of the week in the computer lab completing this assignment. Remember the deadline is this Friday at 2:40 pm.

12.19.12 Hydroponics Project Write Up

For all 4 classes:

Your objective a to collect and analyze the results of your hydroponics project in order to write up your final draft due Friday.

We worked in the computer lab to complete your paragraphs and line graph using the resources found on Hydroponics Links from the page above. You will spend the rest of the week in the computer lab completing this assignment. Remember the deadline is this Friday at 2:40 pm.

12.18.12 DNA Extraction and Introduction to DNA

For periods 1, 3, and 5 your objective was to extract and analyze DNA from strawberries. We started the lesson with mRNA com pairs. Then we went through the background and procedures for the Strawberry DNA Extraction Lab mod. Strawberries have 28 chromosomes with 8 copies of each chromosome! We have 23 chromosomes with 2 copies of each chromosome. That makes it much easier to see something so small as DNA, there is so much of it, just like  it is easier to see someone’s hair color if they have a full head of hair as compared to one or two strands of hair on their head. Once we got through the background you worked with a partner to mash the strawberries to separate the cells and added the buffer to break the cells open or dissolve the cell membrane: photo 6 photo 7 Then you filtered the mixture to separate the DNA from the rest of the cellular materials: photo 8 photo 10 photo 1 District 13-20121218-00200 Then you added the filtered material to the ice-cold alcohol to isolate the DNA and used the pipette to spool the DNA out of solution: photo 12 photo 5 photo 4 photo 3 photo 2 District 13-20121218-00215 District 13-20121218-00214 District 13-20121218-00213 District 13-20121218-00208How cool is that! Did it look like you expected? Do you think we could extract DNA for other food items? What makes you think that? Once you cleaned up you worked with your partner to complete the analysis questions. If you did not turn this in during class you need to finish it for homework, it is due tomorrow. Remember we be meeting in the computer lab 216 for the rest of the week to complete your hydroponics project 2012 with the hydro graph template which is DUE THIS FRIDAY.

For period 6, your obejctive was to describe the structure, relationship and function of DNA and genes. We started by discussing what we remember and chromosomes and DNA . Then we heard from Tim and Moby describing genetics and DNA (sligoms, brainpop). After discussing this information you had an opportunity to illustrate and describe the relation among the terms cell, nucleus, chromosome, gene, and DNA using Genetic Cellular Organization Graphic Organizer an your textbook: Science Explorer: Cells and Heredity . If you finished this assignment you went on to illustrating the structure of DNA in DNA Coloring also using your textbook, Science Explorer: Cells and Heredity . You do not have any homework.

12.17.12 Forensics in the Field, Microarray Simulation, Gel Electrophoresis

Good afternoon! I hope you had a great concert this morning, I’m sure the elementary school enjoyed it.

HOMEWORK for periods 1, 3, and 5: You will be in the computer lab Wednesday, Thursday, and Friday of this week to complete your hydroponics project 2012. Remember your 3 paragraphs and graph are due this Friday, December 21. This is the deadline. If need graph paper, use this hydro graph template. Remember you need to graph all the data we collect on our plants growth on ONE GRAPH. See me if you have questions BEFORE FRIDAY!

For periods 1 and 3 we watched Forensic Detectives: Mysteries and Solutions (schneiderdf, Student) and discussed which techniques we have used in class and the limitations of collecting and analyzing evidence. You do not have any homework due tomorrow.

For period 5, your objective was to describe how turned on and off by simulating a microarray. We started the lesson off with mRNA com pairs. Then we went over example 3 of the Microarray Simulations. You worked in pairs to complete this and turn it in. The rest of the class we watched DragonflyTV: Crime Scene and Mystery Castle: Forensics. You do not have any homework due tomorrow.

For period 6, your objective was to verify their chromatography results by analyzing the gel electrophoresis lab. We started the lesson off with unscramble 2. Then you moved into your groups to collect your data from your hydroponics project for the last time. Then we finished up on the analysis questions from the Gel Electro 2012 6th. You then used this information to complete the Gel Electrophoresis Mini Quiz both of which you turned in. You do not have any homework.

12.13.12 Microarray Simulation and Gel Electrophoresis

Hello! I wish all our performers good luck tonight! Break a leg!

For periods 1, 3, and 5 your objective was to describe how turned on and off by simulating a microarray. We started the lesson off with C SR DNA Quiz. Then watched Brainpop: DNA and RNA (sligoms, brainpop) to review the complementary base pairs. Then we worked with a partner to complete the Examples 1 and 2 of the Microarray Simulations. You then saw how mRNA TRANSCRIBES DNA (reads and creates the complementary base pair sequence in this video:   Remember that Adenine pairs with Uracil in mRNA, this is the only difference, the rest of the complementary pairs are the same as DNA, Thymine pairs with Adenine, Guanine pairs with Cytosine, and Cytosine pairs with Guanine. Whenever scientists are looking for the presence of a specific gene they tag that mRNA sequence with a flourescent dye or marker. That way they have visual evidence that a gene is turned on. This is what we will be doing in our microarray lab, we will be testing to see if a gene for salmonella has been expressed or turned on in salad ingredients. If we see a color change we will know that the salad ingredient has been exposed to salmonella! Period 1 you have no homework, Periods 3, and 5 your homework is Example 3 and it is due Monday.

For period 6 your objective was  to analyze your results of the gel electrophoresis lab in order to find the factory processing the contaminated salad in Sligo Town. You started the lesson off with Cause and effect of Gel Electrophoresis which you needed to use to complete the analysis questions from the Gel Electro 2012 6th. Remember to answer all questions and questions 3-6 require you to list the COLORS of the pigments that have those properties. Here is an example of your results from this experiment:IMG_0348  Then you were able to work together to complete the Gel Electrophoresis Mini Quiz. This cannot be retaken so you needed to confirm your results before turning it in. You do not have any homework.

12.12.12 Advances in Genetics, Microarray Simulation and Gel Electrophoresis

Hello!
For periods 1, 3, and 5 your objective was to describe how turned on and off by simulating a microarray. We started the lesson with separating mixtures 4. Then went back to page 2 of the DNA Coloring. We discussed the video segment Greatest Discoveries:Genetics, The Double Helix (schneiderdf, Student) to help complete the notes on the on the bottom of page 2. If you are interested in learning more about Rosalind Franklin check out this link: Science Women: Rosalind Franklin. Then we went onto discussing how it is possible that cells like this nerve cell:     and this white blood cell: have the exact same genes look and act very differently. The environment and presence of certain ingredients can turn genes on or off. We can use this information to identify which type of salad green was contaminated with salmonella. You then worked with a partner to complete the first page of the Microarray Simulations and saw how scientists measure the kind and amount of mRNA using a microarray:microarray chip . This is one of the tests I ran during my research experience at NIST this summer as well. Check out this link to read about the how I used this technique: Picogreen DNA assay and data analysis. You do not have any homework tonight.

For 6th period, your objective was to verify their chromatography results by conducting the gel electrophoresis lab. We went straight into lab since it was a timely experiment. We started by going over how Gel electrophoresis works. I explained how the wells were created in the gels: all gels gels and how to transfer the samples to the wells:

loading gels 1 loading gels 2 loading gels 3 loading gels 4 loading gels 5

loading gels 6 loading gels 7 by following the procedures from the . After letting the electricity run through your samples for around 12 minutes your results should look like this: gel results great gel results 2 great gel results gel results Can you confirm which factory matches the contaminated salad packaging? We will review the results tomorrow.